1.
Springer Protocols Handbooks
; : 101-113, 2022.
Artículo
en Inglés
| EMBASE | ID: covidwho-2173506
RESUMEN
Bovine coronavirus (BCoV) is an economically significant cause of enteric and respiratory diseases in cattle throughout the world. BCoV is a known cause of neonatal calf diarrhea, winter dysentery in adult cattle, and respiratory disorders in cattle of all ages. In this chapter, we describe a simple and efficient protocol for total nucleic acids extraction to be used in conventional RT-PCR assay. This is a technique used routinely in our virology laboratory to detect BCoV from stool and nasopharyngeal samples of cattle. Copyright © Springer Science+Business Media New York 2016.